Literature review, done in 30 seconds.

Researca reads real academic papers, ranks them by relevance, and synthesizes findings where every claim links to its source.

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You ask

Researca reads 24 papers in full…

Researca ranks them by relevance

And synthesizes one answer — with citations

Genome-wide profiling of CRISPR-Cas9 off-target activity

Tsai et al. · Nat. Biotechnol. · 2015

97

High-fidelity Cas9 variants with minimized off-targets

Kleinstiver et al. · Nature · 2016

95

GUIDE-seq for unbiased genome-wide off-target detection

Tsai et al. · Nat. Biotechnol. · 2015

92

Anti-CRISPR proteins constrain off-target cleavage

Shin et al. · Sci. Adv. · 2017

88

High-fidelityCas9variants[2]andanti-CRISPRproteins[4]cutoff-targetcleavagebyupto40%,whileGUIDE-seq[3]verifiestheremainingeditsgenome-wide[1].

[1] Tsai et al. · Genome-wide profiling of CRISPR-Cas9 off-target activity. Nat. Biotechnol., 2015.

[2] Kleinstiver et al. · High-fidelity Cas9 variants with minimized off-targets. Nature, 2016.

[3] Tsai et al. · GUIDE-seq for unbiased genome-wide off-target detection. Nat. Biotechnol., 2015.

[4] Shin et al. · Anti-CRISPR proteins constrain off-target cleavage. Sci. Adv., 2017.

And synthesizes one answer — with citations

…engineered variants reduced off-target indels ~40%…[2]
…GUIDE-seq detected cleavage genome-wide…[3]
…anti-CRISPR proteins limited residual activity…[4]

High-fidelityCas9variants[2]andanti-CRISPRproteins[4]cutoff-targetcleavagebyupto40%,whileGUIDE-seq[3]verifiestheremainingeditsgenome-wide[1].

[1] Tsai et al. · Genome-wide profiling of CRISPR-Cas9 off-target activity. Nat. Biotechnol., 2015.

[2] Kleinstiver et al. · High-fidelity Cas9 variants with minimized off-targets. Nature, 2016.

[3] Tsai et al. · GUIDE-seq for unbiased genome-wide off-target detection. Nat. Biotechnol., 2015.

[4] Shin et al. · Anti-CRISPR proteins constrain off-target cleavage. Sci. Adv., 2017.

Forty papers. One trustworthy answer.
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